منابع مشابه
Piezo-actuated Mouse ICSI (Intracytoplasmic sperm injection)
Intracytoplasmic sperm injection (ICSI) is not only used in human assisted reproduction (ART), but it is also widely employed for veterinary in vitro fertilization during rare species preservation, germ-line rescue of transgenic animals or for any veterinary assisted conception. Piezo-assisted ICSI, which was first described in 1995 [1], can be employed for assisted conception in animals in whi...
متن کاملRescue ICSI of unfertilized oocytes after IVF.
BACKGROUND Failed fertilization after IVF occurs in 10-20% of cycles. Conflicting results of rescue fertilization by ICSI have been reported. We therefore compared the success rate in terms of fertilization and pregnancy of cycles in which rescue ICSI was performed with those from a matched control group of primarily ICSI cycles. METHODS Unfertilized oocytes from IVF cycles with total fertili...
متن کاملGeometric characterization of cell membrane of mouse oocytes for ICSI.
Intracytoplasmic sperm injection (ICSI) is a broadly utilized assisted reproductive technology. A number of technologies for this procedure have evolved lately, such as the most commonly utilized piezo-assisted ICSI technique (P-ICSI). An important problem with this technique, however, is that it requires a small amount of mercury to stabilize the tip of the penetration micropipette. A complete...
متن کاملHuman Sperm Aster Formation and Chromatin Configuration in Rabbit Oocytes Following Intracytoplasmic Sperm Injection Using a Piezo-Micromanipulator
In human fertilization, the sperm centrosome nucleates a radial array of microtubules called the sperm aster. The sperm aster is responsible for apposition of male and female pronuclei, and later gives rise to the first meiotic spindle. The objective of this study was to determine microtubule assembly and chromatin configuration in rabbit oocytes following intracytoplasmic injection with human ...
متن کاملEffect of calcium ionophore on unfertilized oocytes after ICSI cycles
BACKGROUND Fertilization failure is one of the most problems in assisted reproduction technology (ART). OBJECTIVE The aim of this study was the evaluation of oocytes activation by addition of calcium ionophore in unfertilized oocytes in ICSI cycles. MATERIALS AND METHODS This study was done on 15 ICSI cycles (stimulated with standard long protocol). Mature retrieved oocytes with normal morp...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2021
ISSN: 1940-087X
DOI: 10.3791/60224